- Large scale Int purification (3-100 liters) [PDF]
- Small scale Int purification (0.25-3 liters) [PDF]
- IHF purification protocol [PDF]
- Xis purification protocol [PDF]
- Western protocol [PDF]
- Int recombination and bandshift buffers [PDF]
- Making electrocompetent cells [PDF]
- Buffers [PDF]
- PFGE PDF]
- MIC [PDF]
- Microscopy [PDF]
- TUNEL DNA Damage Detection Assay (updated) [DOC]
- 96 Well Template (updated) [XLS]
- Patching Grid (updated) [PPT]
- Small scale electroporation [PDF]
- Viability Experiment [PDF]
- Antibiotic Information (updated) [DOC]
- 6X Loading Dye for DNA [DOC]
- Alkaline lysis plasmid miniprep [DOC]
- B-Galactosidase assay - Microtiter plate format [DOC]
- Genomic DNA preparation (CTAB method) [DOC]
- Chemically-Competent Cell Recipe [DOC]
- EBU plates [DOC]
- Electrocompetent cells [DOC]
- Protocol for removing markers flanked by FRT sites [DOC]
- NCE Medium [DOC]
- P1 Transduction (E. coli) [DOC]
- DNase-free RNase [DOC]
- Sam's Red Swap Guide [DOC]
- Arbitrary PCR [DOC]
- 4X SDS-PAGE Sample Loading Buffer [DOC]
- Pfx50 Polymerase Formula [XLS]
- Taq Formula [XLS]
If you're having trouble opening or downloading these files, click here